We are starting this series off with FlowJo, which has become very popular, in particular among many in the immunology world who may have large complex datasets. In this series of articles we are going to be looking at a range of software that is available to you for your cytometric analysis and give you the low down on each one, highlighting the strengths and weaknesses so you can make the decision of which software is best for you. So where we use the dedicated software aligned to the analyser for acquisition, we frequently use alternative programs for offline analysis.Īdvantages may be that such software is much more user-friendly for large scale analysis rather than the instrument-linked software (although there are usually analysis tools here too), and it can unify the analysis approach and presentation, so irrespective of what user or cytometer, the data “looks” the same.īut with the variety of software available how do you know which one is right for you? This can be increasingly complex depending on the number of cells, populations, parameters, and combinations. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® NC-3000™ a safe option.Generally, once you’ve acquired your samples on a cytometer, the hard (but hopefully fun!) part of making sense of all the data begins. Used with the disposable Via1-Cassette™, this closed cell counting system helps you stay protected when handling samples. This advanced image cytometer ensures flow-like analysis with high reproducibility, operates with low sample volumes, and is calibration-free. You can view data in plot graphs and interlinked images, which can be saved for later on-demand analysis. The accompanying NucleoView ™ software is ideal for intuitive data analysis and has unlimited licenses. With the FlexiCyte™ license, you can create protocols with options for light source, emission filters and other detailed settings. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and the standard cell count and viability assay. The instrument performs high-speed plug-and-play analytical assays of mammalian, yeast and insect cells.
The NucleoCounter® NC-3000™ advanced image cytometer utilizes fluorescence imaging to characterize cell properties.
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